Separation of cancer cells from white blood cells by pinched flow fractionation
نویسندگان
چکیده
منابع مشابه
Separation of cancer cells from white blood cells by pinched flow fractionation.
In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell types. Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. CTCs are rare cells in blood, but they are important for the understandin...
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Pinched flow fractionation is shown to be an efficient and selective way to quickly separate particles by size in a very polydisperse semi-concentrated suspension. In an effort to optimize the method, we discuss the quantitative influence of the pinching intensity in the balance between the requirements of selectivity and minimal dilution.
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In this study, neural cell sorting of high throughput and high purity is made using two-step separation by combining soft inertial microfluidics and pinched flow fractionation. Both finite element analysis tool and equivalent electric circuits modeling were used to design the microfluidic devices. Separation between neurons and glia cells from rat’s spine cord was demonstrated. To minimize the ...
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We present a computational investigation of the mechanism governing size-based particle separation in microfluidic pinched flow fractionation. We study the behavior of particles moving through a pinching gap (i.e., a constriction in the aperture of a channel) in the Stokes regime (negligible fluid and particle inertia) as a function of particle size. The constriction aperture is created by a pl...
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A concept of "pinched flow fractionation" for the continuous size separation and analysis of particles in microfabricated devices has been proposed and demonstrated. In this method, particles suspended in liquid were continuously introduced into a microchannel having a pinched segment and were aligned to one sidewall in the pinched segment by another liquid flow without particles. The particles...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2015
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c5lc01014d